Hemoglobin Heme Conformation in Patients with Different Oxygen Saturation Values

Alexander I. Yusipovich
Lomonosov Moscow State University, Russian Federation

Elvin S. Allakhverdiev
Russian National Medical Research Center of Cardiology, Moscow, Russian Federation

Evgeniia Yu. Parshina orcid (Login required)
Lomonosov Moscow State University, Russian Federation

Sergey K. Pirutin
Lomonosov Moscow State University, Russian Federation
Shenzhen MSU–BIT University, PRC

Margarita A. Silicheva
Lomonosov Moscow State University, Russian Federation

Oleg V. Rodnenkov
Russian National Medical Research Center of Cardiology, Moscow, Russian Federation

Tamila V. Martynyuk
Russian National Medical Research Center of Cardiology, Moscow, Russian Federation

Georgy V. Maksimov
Lomonosov Moscow State University, Russian Federation
University of Science and Technology MISIS and MISIS University, Moscow, Russian Federation

Paper #3529 received 14 Sep 2022; revised manuscript received 11 Nov 2022; accepted for publication 27 Nov 2022; published online 13 Dec 2022.

DOI: 10.18287/JBPE22.08.040505


The characteristics of prosthetic group of hemoglobin – heme at different values of the oxygen saturation (sO2) was studied in human whole blood of patients with various clinical forms of coronary heart disease using the method of Raman spectroscopy. Correlation between the values of ratios of Raman spectra bands (1375 and 1355 & 1588 and 1552 cm–1, correspondingly) and oxygen saturation was shown. Based on the analysis of the Raman spectra, it is directly shown that at low sO2 the streatching of the heme ring comes before the binding of the bivalent iron atom to the oxygen molecule. It is probably caused by the cooperative effect (the attachment of a ligand to one heme leads to a change in of the protein and therefore the conformations of other hemes without ligands).


Raman Spectroscopy; whole blood; erythrocytes; heme; oxygen saturation

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