Phase Retardation for Dehydrated Human Tears Fluid Samples in Diabetes Mellitus

Daniel A. Chaburkin (Login required)
Samara National Research University, Russian Federation

Svetlana P. Kotova
Lebedev Physical Institute of the Russian Academy of Sciences, Samara Branch, Russian Federation

Anatoly A. Devyatkin
Derzhavin Tambov State University, Russian Federation
T. I. Eroshevsky Samara Regional Clinical Ophthalmological Hospital, Russian Federation

Alfiya Iskhakova
T. I. Eroshevsky Samara Regional Clinical Ophthalmological Hospital, Russian Federation
Samara State Medical University, Russian Federation




DOI: 10.18287/JBPE25.11.040302

Abstract

New analytical capabilities are reported for the V. N. Shabalin − S. N. Shatokhina wedge-shaped dehydration method in the study of biochemical mechanisms of eye diseases development under diabetes mellitus, based on the value of phase retardation of dehydrated samples of human tear fluid. The phase retardation was measured for three sample groups of patients: examined and recognized as healthy patients, patients with undetermined diagnosis of diabetes mellitus, patients with confirmed diagnosis of type 2 diabetes mellitus. The proposed new methodological approach enriches the known morphological methods for the analysis of dehydrated samples of various biological fluids with quantitative parameters over the entire sample area. The approach might prove useful in the research practice of medical and biological fields.

Keywords

wedge-shaped dehydration; tear fluid; phase retardation; phase distribution visualization

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